Whole-Brain Imaging of Neuronal Activity in Zebrafish
Published in Scanners and Imaging.
Whole-Brain Imaging of Neuronal Activity in Zebrafish
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Scientists at the Howard Hughes Medical Institute studied live zebrafish larvae using a novel approach that combined genetics, calcium imaging, and light-sheet microscopy to observe whole brain activity.
This method allowed scientists to record the activity of more than 80% of the 100,000 neurons in the zebrafish brain at single-cell resolution.
Here is how it works (from journal Nature):
The imaging system involves using genetically engineered zebrafish.
The fish’s neurons make a protein that fluoresces in response to fluctuations in the concentration of calcium ions, which occur when nerve cells fire.
A microscope sends sheets of light rather than a conventional beam through the fish’s brain, and a detector captures the signals like a viewer watching a cinema screen.
(Video Credit: Misha Ahrens & Philipp Keller, Howard Hughes Medical Institute)
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